1. No category

オリゴヌクレオチド d(T)2-20のLC/MS分析
LC/MS analysis of oligonucleotides d(T)2-20
F060118A
Oligodeoxythymidylic acid
[d(T)2-20]
A) UV
100
T20
T10
Diode Aray
269 nm
5.50e4
%
T2
0
0
5
10
15
20 min
B) MS
100
Scan ESTIC
7.50e7
T10
T20
T2
%
0
0
5
Column
10
15
20 min
: Hydrosphere C18 (3 mm, 12 nm)
50 X 2.0 mmI.D.
Eluent
: A) 5 mM di-n-butylamine - acetic acid (pH 6.0)
B) 5 mM di-n-butylamine - acetic acid (pH 6.0) / methanol (20/80)
42-70%B (0-20 min)
Flow rate
: 0.2 mL/min
Temperature : 35 °C
Detection
: A) UV at 269 nm
B) ESI negative-mode
Injection
: 5 mL (5 nmol/mL)