1. No category

血清中のバルビツール酸系薬物
Barbiturates in human serum
F100621B
1.
A) Standard (10 µg/mL)
O
H
N
O
H3C
mAU
O
2.
O
H3C O
H3C
O
Pentobarbital
H
N
O
5.
O
H
N
2
H3C
NH
O
3
4 5
Secobarbital
CH3
3.
O
N
H3C
0
H3C O
H3C
Phenobarbital
5
O
H2C
NH
10
O
NH
Barbital
1
H
N
H3C
NH
CH3
15
4.
O
NH
F100531A
0
2
4
6
min
O
Hexobarbital
B) Spiked human serum
Solid-phase extraction method
mAU
YMC Dispo SPE C18
100 mg/1 mL
Condition
2 mL methanol
2 mL water
15
1
10
Load
2
500 µL spiked human serum solution
(each 10 µg)
5
3 4 5
Elute
500 µL methanol/water (85/15)
0
F100531B
0
Column
Eluent
Flow rate
Temperature
Detection
Injection
2
4
6
min
: YMC-Triart C18 (5 µm, 12 nm)
50 X 2.0 mmI.D.
: A) 20 mM HCOONH4-NH3 (pH 9.5)
B) methanol
0-90%B (0-7 min)
: 0.2 mL/min
: 25℃
: UV at 240 nm
: 1 µL
Dilute
500 µL 20mM ammonium formate buffer (pH 9.5)